Effect of ovarian cancer ascites on SKOV-3 cells proteome: new proteins associated with aggressive phenotype in epithelial ovarian cancer

Table 2 Deregulated proteins identified by MALDI-TOF in SKOV-3 cells by effect of EOC ascites (n = 5)

Spot No.		Protein Name	Gene Name	Uniprot ID	NCBI/ SwissProt Accession #	Mw (kDa)	Sequences Matched	Mascot Score	Regulation (Folds)	SD
Upregulated proteins	1	PDZ domain containing protein 11	PDZD11	Q5EBL8	PDZ11_HUMAN	16.1	7	64	+ 2.5	1.5
	2	GTP-binding nuclear protein Ran	RAN	P62826	gi\|48,734,884	24.6	9	90	+ 2.8	0.51
	3	Zinc finger protein 268	ZNF268	Q14587	gi\|14,579,579	92.6	11	68	+ 2.5	0.62
	4	Succinyl-CoA:3-ketoacid-coenzyme A transferase1, OXCT1	N/A	B7Z609	gi\|363,981,034	36.8	5	72	+ 3.2	1.2
	5	Collagen alpha 6(VI) chain	COL6A6	A6NMZ7	gi\|767,925,744	183.4	10	68	+ 2.8	0.05
	6	Actin B	ACTB	P60709	gi\|15,277,503	40.5	9	103	+ 2.4	0.78
	7	Keratin, type II cytoskeletal 8	KRT8	P05787	gi\|119,617,058	53.4	11	75	+ 2.3	0.83
	8	Synaptotagmin-like protein 3	SYTL3	Q4VX76	SYTL3_HUMAN	70	8	64	+ 2.5	0.52
	9	Vimentin	VIM	P08670	gi\|47,115,317	53.6	9	79	+ 2.7	0.12
Downregulated proteins	10	Human Leucocytic Antigen I	HLA-I	P01892	gi\|326,416,438	21.7	6	79	- 2.3	0.11
	11	Purine nucleoside phosphorylase	PNP	P00491	gi\|37,926,571	32.2	7	69	- 2.1	0.38
	12	Prelamin-A/C	LMNA	P02545	gi\|767,909,266	61.6	9	69	- 2.4	0.76
	13	Actin, cytoplasmic 2	ACTG1	P63261	gi\|4,501,887	42.1	13	97	- 2.2	0.99
	14	Heterogeneous nuclear ribonucleoprotein H1	HNRNPH1	P31943	gi\|767,938,360	50.5	7	75	- 2.5	1.0
	15	Heat shock protein beta-1	HSPB1	P04792	gi\|662,841	22.4	7	91	- 2.9	0.88
	16	ADP ribosylation factor	ARF1	P84077	gi\|545,719,724	44.6	8	69	- 2.4	0.09
	17	Lon protease mitochondrial	LONP1	P36776	gi\|414,046	95.5	10	87	- 3.0	1.5
	18	Peroxiredoxin-2	PRDX2	P32119	gi\|32,189,392	18.4	5	85	- 2.6	0.48
	19	Cathepsin D	CTSD	P07339	gi\|672,886,498	26.7	6	77	- 2.5	0.89
	20	Synaptotagmin-1	SYT1	P21579	gi\|167,744,962	32.5	8	68	- 2.5	1.2

ISSN: 1477-5956