Table 1 Elution gradient table of liquid chromatography for separation of peptide fractions
From: A quantitative proteomics analysis for small molecule Stemazole’s effect on human neural stem cells
| Time (min) | Flow rate (mL/min) | Mobile phase A | Mobile phase B |
|---|---|---|---|
| 0 | 1 | 97% | 3% |
| 10 | 1 | 95% | 5% |
| 40 | 1 | 80% | 20% |
| 68 | 1 | 60% | 40% |
| 70 | 1 | 30% | 70% |
| 74 | 1 | 0 | 100% |
| 90 | 1 | 0 | 0 |